bronchial tracheal epithelial cells pbec  (ATCC)

Journal: Frontiers in Immunology

Article Title: Discovery and Use of Long dsRNA Mediated RNA Interference to Stimulate Antiviral Protection in Interferon Competent Mammalian Cells

doi: 10.3389/fimmu.2022.859749

Figure Lengend Snippet: Primary Bronchial Epithelial/Tracheal Cells (pBECs) pre-soaked with long dsRNA of viral genes inhibits infection with corresponding viruses. The pBECs were grown to confluence and were shown to exhibit characteristics indicative of epithelial/tracheal cells, including mucous production and cilia function (A) . The pBECs were pre-soaked with either DPBS, 500 ng/mL of the mis-matched mCherry dsRNA control or 500 ng/mL of VSV N protein dsRNA before infection with VSV-GFP (MOI = 0.1) for 24h (B) . The pBECs were also pre-treated with either DPBS, 50 μg/mL of HMW pIC, 500 ng/mL of the mis-matched mCherry dsRNA control or 500 ng/mL of HCoV-229E M protein dsRNA before infection with HCoV-229E (MOI = 0.1) for 24h (C) . Error bars represent +SEM, and each data point represents the average of 3 independent replicates. A p-value of less than 0.05 was considered to be statistically significant. Error bars with different letters represent significantly different data.

Article Snippet: Normal human primary bronchial/tracheal epithelial cells (pBECs) were purchased from ATCC (PCS-300-010).

Techniques: Infection, Control